Detection of citrus vein phloem degeneration disease (Candidatus Liberibacter asiaticum) in orange cv. Selayar, Citrus reticulata L.

“Candidatus Liberobacter asiaticum” and “Candidatus Liberobacter africanum,” the Two Species Associated with Citrus Huanglongbing (Greening) Disease

16S/23S intergenic spacer regions from the rRNA operons of two strains of “Candidatus Liberobacter asiaticum” and one strain of “Candidatus Liberobacter africanum” were cloned and sequenced. The intergenic spacers of the two “Candidatus L. asiaticum” strains studied are identical and contain the genes for isoleucine tRNA (tRNA”‘) and alanine tRNA (tRNAMa) separated by 11 nucleotides. The interg...

Genetic Mechanisms of the Devious Intruder Candidatus Liberibacter in Citrus

Response of sweet orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' infection: microscopy and microarray analyses.

Citrus greening or huanglongbing (HLB) is a devastating disease of citrus. HLB is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium 'Candidatus Liberibacter spp.' In this report, we used sweet orange (Citrus sinensis) leaf tissue infected with 'Ca. Liberibacter asiaticus' and compared this with healthy controls. Investigation of the host response was examined with ...

Quantitative distribution of 'Candidatus Liberibacter asiaticus' in citrus plants with citrus huanglongbing.

Citrus huanglongbing (HLB), or greening disease, is strongly associated with any of three nonculturable gram-negative bacteria belonging to 'Candidatus Liberibacter spp.' 'Ca. Liberibacter spp.' are transmitted by citrus psyllids to all commercial cultivars of citrus. The diseases can be lethal to citrus and have recently become widespread in both São Paulo, Brazil, and Florida, United States, ...

Leaf-disc grafting for the transmission of Candidatus Liberibacter asiaticus in citrus (Citrus sinensis; Rutaceae) seedlings1

PREMISE OF THE STUDY The search for resistance/tolerance to the devastating citrus huanglongbing disease (syn. HLB or citrus greening) is generating an increasing number of new plants of diverse genetic makeup. As the increasing number of new plants require more space, resources, and time, the need for faster and more efficient HLB screening tests becomes crucial. METHODS AND RESULTS The leaf...